Adenine base editors optimized for rice
Chinese scientists improved on previous advances in the field of single-base editors. This technology, used to truly precisely edit genomes, is still bothered by efficiency issues. In rice, researchers were able to modify 16-59% of organisms, depending on targeted site. Now, new experiments show that the efficiency can be increased by up to 4.5 times.
Previous plant base editor was called pHUN411-ABE. The team modified it by adding a new sgRNA and fusing it with hygromycin phosphotransferase. New editor, named pPUN411-ABEH, was more efficient, thanks to higher expression and better selection process. According to the brief report, 97.9% of all plants were genetically modified at all targeted sites.
The study proves that near-complete efficiency can be achieved in genetic engineering when it comes to economically important organisms – rice plants.
Publication: Li, J. , Qin, R. , Zhang, Y. , Xu, S. , Liu, X. , Yang, J. , Zhang, X. and Wei, P. (2019), Optimizing plant adenine base editor systems by modifying the transgene selection system. Plant Biotechnol J. doi:10.1111/pbi.13304