BAM15 gene linked to obesity
BAM15’s function is not yet known, but it may be involved in regulating energy metabolism and energy expenditure. In the future, we hope to use BAM15 to treat obesity and diabetes.
But why would this mitochondrial uncoupler have a beneficial effect on the body? To answer this question, we first need to know more about how mitochondria function. Mitochondria are tiny organelles inside cells that perform a variety of essential cellular functions. In humans, mitochondria are composed of just over 30 proteins. In addition to their essential role in metabolism, mitochondria are involved in regulating other important processes such as immune response, inflammation, and cell death.
Most importantly, mitochondria play a critical role in energy metabolism. A mitochondrion contains an enzyme called cytochrome c oxidase, which is responsible for converting the energy from food into ATP, the energy-storing molecule in all cells. This complex process is essential for normal cell function. However, if the cytochrome c oxidase enzyme is impaired, the mitochondria may not be able to produce enough ATP.
In order to maintain energy homeostasis, mitochondria must be able to regulate their own energy production. This is accomplished by pumping out ATP. The action of this pump is regulated by the release of a molecule called AMP from the mitochondrial matrix. AMP is a precursor to the production of a protein called adenine nucleotide (ADP). This protein is the key to regulating energy metabolism.
To test the hypothesis that the BAM15 uncoupler would have a beneficial effect on energy metabolism, we first measured the effect of BAM15 on the ATP production rate of three cell lines. The three cell lines were PIK3CA, JEG3, and MDA-MB-231. We also tested the effect of BAM15 on the AMP/ATP ratio in the mitochondrial matrix.