Designed transposase makes CAR-T cells

Transposase “Sleeping Beauty” was discovered and used within a transposon system in the 90′ to insert genes into genomes, but it hasn’t gained much interest over years. The method was hard to control, it introduced multiple unwanted mutations and overall toxicity. After twenty years, a new version of Sleeping Beauty aims to improve the enzyme.

Team of scientists from German institutions made hsSB – high solubility Sleeping Beauty – which is stable at various temperatures and has preserved functionality. They tested it in cell lines and in CAR-T therapy for mice. Moreover, they observed spontaneous uptake of transposase system without transfection.

Michael Hudecek, senior author of the study, concluded:

The new transposase and the genome engineering procedures we developed will find direct use in therapeutic cell engineering.

Publication: Querques, I., Mades, A., Zuliani, C. et al. A highly soluble Sleeping Beauty transposase improves control of gene insertion. Nat Biotechnol (2019) doi:10.1038/s41587-019-0291-z
Photo: National Center for Microscopy and Imaging Research (NCMIR)

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